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Ultra-high resolution like sub-2μm columns
Faster separation like sub-2μm columns
Lower back pressure than sub-2μm columns resolve chemically similar analytes.
Compatible with exsisting HPLC or LC/MS.

Vertical UPS HPLC COLUMN Vertical UPS HPLC COLUMN

    VertiSep™ Integral columns are made with superficially porous silica microsphere (Core-shell) technolohy. The packings consist of ultra-pure silica in solid cores and thin outer shells with uniform pores. These offer excellent kinetic properties providing stable, ultra-high resolution and faster than HPLC column with conventional packings.

    VertiSep™ Integral columns are 2.6μm in particle size, very narrow particle size distribution and the higher particle density. This provides efficiency of about at least 20,000 in which comparable to sub-2μm columns.

    VertiSep™ Integral particles have a solid core of 1.7μm and 450nm thick outer shell respectively. The thin outer porous shell allows rapid solute mass transfer (fast kinetics) so that mobile phase flow rate can be increased to allows for very fast separations without sacrificing resolution like sub-2μm columns.

    As only the thin outer shell of core shell particle is porous. This allows less back pressure and rapid solute diffusion in and out of the thin porous shell comparing to totally porous sub-2μm particle. VertiSep™ Integral columns limit at pressure up to at least 5,000psi that are compatible with standard HPLC and LC/MS equipments.

     All totally porous particles (1.8μm, 3μm, and 5μm) have an acceptable particle size distribution. However, VertiSep™ Integral core- shell particle has a narrow particle size distribution, which reduces multi-path diffusion and results in high efficiency.

 
Vertical UPS HPLC COLUMN

Vertical UPS HPLC COLUMN


Column : 3.0 x 100mm
Mobile phase : Acetonitrile/water
Mobile phase : (60/40)
Flow rate : 0.5mL/min
Injection : 3μL
Temperature : 25 ℃
Detection : UV 254nm
Analytes : Toluene

    The Van Deemter shows that the smaller (2.6μm) particle size of VertiSep™ Integral particles results in signifi cantly higher effi ciency for toluene than 5μm and 3μm particle columns. The less increase in plate height with mobile phase velocity increase for the VertiSep™ Integral particles is also in keeping with the smaller particle size and the high kinetic properties of core shell structure.
    In comparison with sub-2μm column, VertiSep™ Integral shows very similar effi ciency; however, but less backpressures that fl ow rates can be increased to speed up analysis.

 

Vertical UPS HPLC COLUMN


Column Column : 3.0 x 100mm
Mobile phase : Acetonitrile/water
Mobile phase : (60/40)
Flow rate : 0.5mL/min
Injection : 3μL
Temperature : 25 ℃
Detection : UV 254nm
Analytes :
    1 Uracil
    2 Phenol
    3 N,N-Diethyl-m-Toluamide
    4 Toluene

    The effi ciency on VertiSep™ Integral column is about more than 90% of sub-2μm columns and about 2 times the effi ency of the 3μm columns.
    The pressure on VertiSep™ Integral column is below 5,000psi while the pressure on sub-2μm column is above 6,000psi. VertiSep™ Integral columns are compatible with all HPLC systems.
 
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